PDF | The preliminary phytochemical analysis of Ephedra alata indicated the presence of cardiac glycosides, reducing sugars, flavonoids. Descriptions and articles about the Ephedra, scientifically known as Ephedra alata in the Encyclopedia of Life. Includes Overview; Distribution; Ecology; Hab. Two new flavonol glucosides have been identified in Ephedra alata, namely, herbacetin 8-methyl ether 3-O- glucosideO-rutinoside and herbacetin.

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Seed samples of Ephedra alata Decne approximately. Initial seed moisture content was 9. The overlaid UV-Vis spectra of the main peaks are depicted a,ata the right corner of chromatogram B. Advances in Biological Research. Effect of various storage containers on seed vigor indexZ of E. Total phenolic content, antioxidant and antibacterial activities of the extract of ephedra procera fisch.

Maintenance of Ephedra alata Seeds Viability via Storage Containers

Ephedra alenda Stapf Andr. The mixture was vortexed for sec.

There are two types of antioxidant assays used to evaluate the antioxidant activity of plant extracts. Purified nitrogen was used as source and exhaust gases. List of Abbreviations and nomenclature: The bleaching of DPPH solution increases linearly with increasing amount of ephedraa in a given volume.

Introduction Ephedra is a medicinal plant belonging to the Ephedraceae family. The plant species is properly authenticated by Professor Khalid Sawalha, the director of biodiversity research laboratory, Al-Quds University.

These types of compounds are very close to isomeric flavonoid glycosides.

Up to our knowledge, this is the first investigation for seedborne mycoflora of E. Results and Discussions In the present investigation, 31 seed samples of E. TFC however was highest in the following order: Antioxidant activity applying an improved ABTS radical cation decolorization assay.

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The HPLC system was then equilibrated for 5 minutes with the initial mobile phase composition prior injecting the next sample. Another peak at a retention time of Healing effect of hydro-alcoholic extract of Ephedra pachyclada Boiss. On other hand, the biotic stresses such as seedborne fungi play an important and vital role in deterioration of seed quality [1,2]. Distilled water 4 mL was added to 1 mL of the extract in a test tube.

Results of a study of Ghasemi et al. The retention of superior SMC recorded here by both polyethylene bags and tin cans is probably at- tributed to impervious nature of previous storage con- tainers compared with either cloth or paper bags [21].

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This wavelength was selected since the main Ephedra alata peaks showed a maximum absorption close to it. The deterioration of plant community had occurred in Saudi Desert due to abiotic factors such as soil salinity [7], ed- aphic factors of the soil [29] and soil epyedra [6]. The color changed from purple to yellow and the absorbance at wavelength nm decreased.

Acta Poloniae Pharmaceutica Drug Research. Nevertheless, similar mycological studies for many other seeds showed that Aspergillus and Fusarium were the most common genera as seedborne fungi [2; 16].

These plants are also used for consumption by animals such as camelidae, bovines and caprines. This parameter accounts for the presence of efficient oxygen radical scavengers, such as phenolic compounds. The fungal colonies developing around. Application of prophylactic fungicides is not the pre. Ehpedra alata is a species of Ephedra. The plant was air-dried in the dark at room temperature for five alat, then milled to a powdered plant material, and then stored in fridge until extraction.


The female, or ovulate cones are found at the nodes of the stems. These plants fibrous roots help to anchor the plant in shifting sand dunes, control sand erosion and are tolerant to saline soils. Received October 22 nd; revised November 19 th; accepted November 22 nd Calibration curve of each individual standard was also prepared at three concentration levels namely 50, and ppm.

Aflatoxins B 1B 2and G 1 were extracted and cleaned up from storage seeds using chloroforme and cleaned usin g column chromatography according to AOAC [10]. Ehpedra alata is a small terrestrial shrub or Chamaephyte. Effect of various storage containers on percentage germination of Ephddra.

Freshly prepared Alat reagent 3. Immediately, the volume of reaction mixture was made to 10 mL with distilled water. There is a correlation between antioxidant activities and total phenolic content but not with total flavonoids content.

These results demonstrated that the Ephedra plant extracts are rich with flavonoids range: The genes Aspergillus was the most predominant and represented by 6 species namely A. Standard curve was prepared using different concentrations of Trolox. Total Flavonoid Content TFC The results of ferric chloride colorimetric test for determining flavonoids content are presented in Table 1. Seeds from this species have been obtained and stored for the Millennium Seed Bank Project because it is unclear if this species population is sustainable with its wide range of uses.