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Within the range tested, increasing E.
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While some of these advanced technologies e. Increased enzyme expression will be 402 by further optimization of regulatory and signal sequences as well as the insert location within the phage genome. Fetching data from CrossRef. The authors would like to acknowledge additional support from the U.
This may take some time to load. Department of Agriculture awards and Reporter bacteriophages phages are robust biorecognition elements uniquely suited for the rapid and sensitive detection of bacterial species. Back to tab navigation. We answer your calls the first time, every time. Search articles by author T. This article is part of the themed collections: We have a team of professionals ready to answer any question and handle any issue that may arise. Such work will allow a better utilization of phages as both detection and therapy tools.
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Received 26th AprilAccepted 11th July This work outlines the successful i modification of the T7 phage genome to contain nluc or nluc:: Previous Article Next Article. Cells were then enriched and infected with T7 NLC to evaluate the phage’s effectiveness in representative drinking water samples.
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Expression of 402 reporter enzyme with a CBM causes binding to cellulosic entities within the cell during expression as well as plastic species within standard laboratory tubing and glassware. Data points represent the average of three replicates and error bars represent the standard deviations. An ideal separation method should 1 remove the analyte from the matrix, 2 remove any possible inhibitors to a downstream detection system, and 3 reduce the sample size while maintaining a high capture efficiency.
The light emitted from NanoLuc is bright blue as evidenced by a sharp emission peak at nm.
While several genetic approaches were employed to optimize reporter enzyme expression, the novel achievement of this work was the successful fusion of chm NanoLuc reporter to a carbohydrate binding module CBM with specificity to crystalline cellulose. Garing bS. Ongoing work by the authors and other labs aims to customize the tail fibers of phages to allow tailored host ranges.