40818 DATASHEET PDF

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Application Dilutions Western Blotting 1: A cell lysate pre-clearing step is highly recommended to reduce non-specific protein binding to the Protein A Magnetic beads.

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Would you like to visit your country specific website? USP8 Antibody – Preparing Cell Lysates Aspirate media. Remove buffer once solution is clear.

Prepare solutions with reverse osmosis deionized RODI or equivalently purified water. Block specimen in Blocking Buffer for 60 min. Isotype controls should be concentration matched and run alongside the primary antibody samples. From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Detection of Proteins Directions for Use: Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film.

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Protein A Magnetic Beads: Pre-wash magnetic beads see Cell Lysate Pre-Clearing section, steps 1 and 2. ATP 10 mM for kinase assays: Separate the beads from the lysate using a magnetic separation rack, transfer the pre-cleared lysate to a clean tube, and discard the magnetic bead pellet. Incubate 30 min on ice. Do not aliquot the antibody. More about how we get our images.

ZL Datasheet pdf – GHz Fixed Modulus Divide 4 Prescaler – Zarlink Semiconductor

Proceed to analyze by western immunoblotting or kinase activity section D. Formaldehyde is toxic, use only 40881 a fume hood. Microcentrifuge for 5 min. The supernatant is the cell lysate.

Phospho-STING (Ser366) (D8K6H) Rabbit mAb #40818

Sample Analysis Proceed to one of the following specific set of steps. Wash 4081 times for 5 min each with 15 ml of TBST. Wash cells by centrifugation in excess 1X PBS to remove methanol. If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.

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Alexa Fluor is a registered trademark of Life Technologies Corporation. Aspirate media from cultures; wash cells with 1X PBS; aspirate.

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Prepare solutions with reverse osmosis deionized RODI or equivalent grade water.

Datasheef studies have shown that USP8 is an essential growth-regulated enzyme indespensible for cell proliferation and survival 3,4. For best results, allow mountant to cure overnight at room temperature. Pre-wash magnetic beads just prior to use:.

Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips. Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Incubate for 30 min at room temperature. Vortex, then microcentrifuge for 30 sec. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available.

Immunoprecipitation Cell Lysate Pre-Clearing Optional A cell lysate pre-clearing step is highly recommended to reduce non-specific protein binding to the Protein A Magnetic beads.

Rinse three times in 1X PBS for 5 min each. Remove PBS and add 0.